Two more people from two unnamed Premier League clubs have tested positive after the second batch of testing.

A total of 996 tests were carried out in the second run of testing, with the number of tests available to each club increased from 40 to 50. All 20 clubs have now carried out two rounds of testing, with clubs due to take part in a third set scheduled on Monday and Tuesday.

A statement on Saturday evening read: “The Premier League can today confirm that on Tuesday 19 May, Thursday 21 May and Friday 22 May, 996 players and club staff were tested for COVID-19. Of these, two have tested positive from two clubs.

“Players or club staff who have tested positive will now self-isolate for a period of seven days.

“The Premier League is providing this aggregated information for the purposes of competition integrity and transparency.

“No specific details as to clubs or individuals will be provided by the League and results will be made public after each round of testing.”

A total of 748 tests were conducted in the first round of testing last Sunday and Monday with six testing positive.

Burnley assistant manager Ian Woan tested positive with Watford having three positive test results for two staff and defender Adrian Mariappa

Watford manager Nigel Pearson announced on Friday that two more of his players had gone into self-isolation after being in close contact with people who have tested positive.

Prior to widespread testing three Brighton players tested positive,  Chelsea’s Callum Hudson-Odoi was also confirmed to have had the virus with Arsenal manager Mikel Arteta one of the first-named in the health crisis.

The results mean there have been 1,736 negative results in the first two rounds, around 99.5 per cent.